In the NSCRC set, univariate Cox regression analysis identified clinical factors significantly associated with OS (Table 4) were sCD4 (p < 0.001), iCD8 (p = 0.002), gender (p = 0.017), pathological T stage (p = 0.001), lymph node metastasis (p < 0.001), TNM stage (p < 0.001), tumor differentiation (p = 0.003), lymphovascular invasion (p < 0.001), tumor deposit (p < 0.001) and tumor budding (p < 0.001). The gene discussed is SCD5; the disease is metastatic malignant neoplasm in the lymph nodes.