Furthermore, in the applications to aging, Covid-19 and colon cancer progression considered here, ELVAR displayed higher sensitivity than Milo and DA-seq to detect biologically important and plausible DA-shifts, such as the age-related shift from naïve to mature Cd4+ T-cells in lung tissue, thus mirroring the corresponding known shift in blood22,24, or the increased epithelial stem-cell and T-regulatory fraction in polyps. This evidence concerns the gene CD4 and malignant colon neoplasm.