RAMP3 expression was detected by RT-PCR in five common laboratory hepatocellular carcinoma cell lines (Huh7, HepG2, SNU387, MHCC97H and Hep3B) (Additional file 5: Figure S5C), followed by overexpression of RAMP3 in Hep3B and HepG2 (Additional file 5: Figures S5D, E), followed by separate CCK-8 proliferation, transwell proliferation, migration and invasion assays on hepatocellular carcinoma cells. This evidence concerns the gene RAMP3 and hepatocellular carcinoma.