In this study, we investigated the effects of exosomes of WJ-MSCs on genes that are involved in liver fibrosis progression, including TGF-β, α- smooth muscle actin (α-SMA), collagen1α genes in the presence or absence of cholesterol in the LX2 cell line (a type of cell derived from HSCs), and also phosphorylation of Smad3 protein level. Here, TGFB1 is linked to Hepatic fibrosis.