To verify whether ASIC1a activation affects the endogenous NPC migration, the model of distal middle cerebral artery occlusion (dMCAO), which has been proved to be a highly reproducible and well-defined infarct model without the confounding effects of reperfusion [24] and widely used in neurogenesis research [25], was established to mimic human stroke. This evidence concerns the gene NPC1 and Stroke.