Next, we carried out several assays to ensure quality of the FTD-hESC line and found a normal karyotype (Supplementary Fig. 1B), normal expression levels of pluripotency markers NANOG and OCT3/4 at mRNA (Supplementary Fig. 1C) and protein levels (Supplementary Fig. 1D), and normal levels of proliferation and cell death (Supplementary Fig. 1E). This evidence concerns the gene NANOG and frontotemporal dementia.