Furthermore, the transcription factors of 4 biomarkers were predicted, and seven transcription factors (GTF2B, MYNN, TAF7, ZNF639, POLR2H, GTF2E2, GTF2A2) that were significantly and differentially expressed in the NASH and NAFL groups were obtained (Figure 6B). This evidence concerns the gene GTF2B and non-alcoholic fatty liver.