Furthermore, the transcription factors of 4 biomarkers were predicted, and seven transcription factors (GTF2B, MYNN, TAF7, ZNF639, POLR2H, GTF2E2, GTF2A2) that were significantly and differentially expressed in the NASH and NAFL groups were obtained (Figure 6B). The gene discussed is GTF2A2; the disease is metabolic dysfunction-associated steatohepatitis.