We discovered that the infiltration abundance of macrophage M2 was dramatically increased in the group with high ACTA2 (p<0.001), FLNA (p<0.001), TAGLN (p=0.002) and TPM1 (p<0.001) expression compared with the low expression group, which demonstrated a clear correlation between the amount of M2 infiltration and the expression of the four genes in the immune microenvironment of bladder cancer. This evidence concerns the gene ACTA2 and urinary bladder cancer.