In addition, the results of western blot showed that the phosphorylation levels of AKT (Ser473), GSK-3 β (Ser9), and ERK1/2 (Thr202/Tyr204) decreased in gallbladder cancer cells after CEP55 knockdown, but the protein expression level of FOXO3a increased in the siCEP55 group (Figure 8B). This evidence concerns the gene AKT1 and gallbladder cancer.