After the transfection of pcDNA3.1/GATA6-AS1 into or the transfection of sh-GATA6-AS1#1/#2/#3 into GC cells, qRT-PCR analysis was applied to test the overexpression efficiency of pcDNA3.1/GATA6-AS1 or silencing efficiency of sh-GATA6-AS1#1/2/3. The gene discussed is GATA6; the disease is gastric cancer.