These results showed clearly that AAV-SaCas9/17-Sg2 effectively restored the retinal function and preserved photoreceptor cells in the humanized RHO mutant mouse model, supporting the potential of AAV-SaCas9/17-Sg2 as an effective drug for RHO-T17M-associated RP. The gene discussed is RHO; the disease is retinitis pigmentosa 1.