Furthermore, after the challenge with the H5N1 virus at 120 days, another peak both for IL-1 and TNF-α (Fig. 9A and F) can be observed: this latter presents a lower height compared to the ones related to the increase of the two cytokines linked to the administration of the vaccine, meaning that this multi-epitope vaccine protects patients against fever after the infection. The gene discussed is TNF; the disease is infection.