CALCA and pachyonychia congenita: To gain insight into how iASPP may suppress oncogenic RAS-driven PC, we performed RNA sequencing (RNA-seq) of PC cell lines with the following genotypes: KC (n = 3), KC;iASPPΔ8/Δ8 (n = 4), KPC (n = 4) and KPC;iASPPΔ8/Δ8 (n = 5) (Fig. 7A).