To further identify potential signaling pathways involved in the regulation of osteogenic differentiation, hPDLSCs pre-treated with IL-6 at 20 ng/mL for 24 h were cultured in OM for 21 days and the expression levels of specific target genes were measured on days 1, 3, 7, 14, and 21 (Fig. 4A). This evidence concerns the gene IL6 and ocular melanoma.