RBFOX3 and Alzheimer disease: Example images showing the extent of neuronal T49-positive tau aggregates in wells treated with AD-tau versus those that were not seeded are shown in Figure 1D, as are parallel AD-tau seeded and non-seeded cultures that were fixed and permeabilized followed by staining with NeuN (34) and MAP2 (35) antibodies to assess the number of neuronal cells and the dendritic process area, respectively.