It is found that serum MANF levels are significantly increased, have independent correlation with admission NIHSS scores, baseline hematoma volumes and post-stroke 90-day mRS scores, are associated with END and poor 90-day prognosis independently, show efficient prognostic predictive potential and exhibit additive effect of prognostic ability when combined with NIHSS scores and hematoma scores. This evidence concerns the gene MANF and hematoma.