Using the multivariate linear regression model, wherein the significantly correlative variables were entered, serum MANF levels were independently correlated with NIHSS scores (beta, 3.912; 95% CI, 1.623-6.200; VIF = 2.394; t = 3.385; P = 0.002) and hematoma volumes (beta, 1.688; 95% CI, 0.764–2.612; VIF = 2.661; t = 3.617; P = 0.001). This evidence concerns the gene MANF and hematoma.