Colon (SW480), pancreatic (PANC1), esophageal (TE7), and gastric (AGS) cancer lines all showed significantly decreased levels of DNA methylation at CpG islands upstream of the LBH promoter compared to either low-tumorigenic (colon: RKO), or normal-derived cell lines (pancreatic: HPNE, esophageal: EPC2, gastric: GES-1) (Fig. 4E). This evidence concerns the gene LBH and cancer.