We next analyzed LBH protein expression and WNT pathway activation by Western blot analysis and TOPFLASH reporter assays in CRC cell lines with no (RKO), low (HCT116 – CTNN1mut), and high (CaCO2, SW480 – APCmut) WNT activation (Fig. 7C, D). The gene discussed is LBH; the disease is colorectal carcinoma.