Having established roles for the gloB-encoded glyoxalase-II on the utilization of glucose and the resistance of Salmonella to H2O2, we tested the virulence of mutants in the methylglyoxal pathway in both bone-marrow-derived macrophages and a chronic granulomatous disease model of Salmonella infection dominated by the antimicrobial activity of the phagocyte NADPH oxidase. Here, HAGH is linked to chronic granulomatous disease.