Multivariate Cox proportional hazard regression analysis showed that the C4 subtype was an independent predictor of the best OS and DFS, whereas the C3 subtype was an independent predictor of the worst OS and DFS after adjustment for age, gender, tumor site, TNM stage, grade, adjuvant chemotherapy or not, MSI status, BRAF and KRAS mutations, and the CMS classification system in the combined cohort (the CRC-AFFY and CRC-RNAseq cohorts) (Supplementary file 1h). The gene discussed is BRAF; the disease is neoplasm.