Through the experiment, the following findings were obtained:SRC, IGF1, IGF1R, HSP90AA1, MAPK1 and MMP9 are high expression in CRC tissues and after the intervention of vitamin D3, the expression of these genes was reduced in cancer tissues, and the differences were statistically significant (p < 0.05). The gene discussed is MAPK1; the disease is colorectal carcinoma.