It was found that the sustained release of DOX caused the ICD of B16F10 cells in vitro, leading to the release of tumor-associated antigens (TAAs) and damage-associated molecular patterns (DAMPs), such as exposure of calreticulin to cell membranes, ATP secretion and high mobility group box 1 (HMGB1) release. Here, HMGB1 is linked to neoplasm.