The top-scoring knockouts with reduced fitness in NCI-HCC cells [(ZNCI-HCC − ZNthy-ori 3-1)/\documentclass[12pt]{minimal}\usepackage{amsmath}\usepackage{wasysym}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{amsbsy}\usepackage{upgreek}\usepackage{mathrsfs}\setlength{\oddsidemargin}{-69pt}\begin{document}$\surd 2$\end{document} < −2] on day 11 were GPX4, GAPDH, GABPA, and G6PD, and on day 16 were GPX4, GAPDH, HSPD1, and G6PD (Fig. 5B; Supplementary Fig. S5A). The gene discussed is GPX4; the disease is hepatocellular carcinoma.