PTEN and hepatocellular carcinoma: Sequencing using anchored multiplex PCR for a panel of over 100 known cancer genes found MGH-HCC1 cells to also harbor biallelic loss of CDKN2A and PTEN and NCI-HCC cells to have truncating nonsense mutations in NF1 and NF2, a hotspot TERT promoter variant (C228T), and partial homozygous losses of CDKN2A and PTEN (Supplementary Fig. S4B).