Upregulated expression of these inflammasome components, except for the scaffold protein ASC and the receptor AIM2, was also confirmed at the protein level in both colonic epithelium and tumors from DSS‐ and AOM/DSS‐treated Smad4‐deficient mice, including Nlrp3, Il‐1β, and cleaved caspase‐1 (Figure 3h,i and Figure S3f,g, Supporting Information). This evidence concerns the gene SMAD4 and infectious otitis media.