As another means of ruling out the p53 pathway as an explanation for altered Cdkn2a expression in Myc; sgKmt2c cells, we tested the ability of MLL3 to regulate Cdkn2a transcripts in an orthogonal liver cancer model driven by Myc and inactivation of Axin1, which is a well-defined tumor suppressor that negatively regulates β-catenin activity in HCC (Satoh et al., 2000). This evidence concerns the gene CDKN2A and hepatocellular carcinoma.