INS and type 1 diabetes mellitus: Our gating strategy more sensitively addresses the fate potential of insulin-binding B cells by eliminating the large (97–99%) competing repertoire of non-insulin-binding B cells that could be recruited into spontaneous and perhaps T1D-irrelevant GCs, whereas the alternative gating strategy is best suited to address the overall composition of GC B cells, which was massively perturbed when anti-insulin 8F10 T cell help was provided (17).