The quantification of phospho-SMAD2/3 and SMAD2/3 expression relative to GAPDH (loading control) in control (n=6) and IPF (n=6) lung fibroblasts treated for 30 min with TGF-β1 after 48 hr transfection with PRRX1 siRNA relative to siControl +TGF-β1 condition (red dashed line), is displayed as dot plot with median on the right. Here, GAPDH is linked to idiopathic pulmonary fibrosis.