Therefore, VitD supplementation may be helpful in the prevention of NAFLD by modulating some serum liver function markers, lipid profile, hepatic redox status, selective molecules involved in the inflammatory and antiinflammatory processes, and mRNA expression levels of regulatory molecules, SREBP-1-c, PPAR-α, and IRS-2 involved in lipogenesis, lipolysis, and insulin signaling, respectively. This evidence concerns the gene IRS2 and metabolic dysfunction-associated steatotic liver disease.