Together with the polyspermy phenotype of Plcz1−/− mice (Hachem et al., 2017; Nozawa et al., 2018) and the infertile male carrying PLCZ1 bi-allelic mutations reported (Peng et al., 2023), we proposed that polyspermy was an important phenotype of PLCZ1-related infertility, suggesting the necessity of genetic test on PLCZ1 gene for infertile males undergoing polyspermy in their previous ART cycles. Here, PLCZ1 is linked to Infertility.