BMI, ferriman-gallway (F-G) score, self-rating anxiety scale (SAS) score, self-rating depression scale (SDS) score, and serum free androgen index (FAI) level were decreased (FAI), while free androgen index (PCOSQ) score, serum SHBG, and β-endorphin levels were increased. Acupuncture treatment of PCOS patients can effectively relieve anxiety and depression. This evidence concerns the gene SHBG and depressive disorder.