Urea-based PSMA-targeting lipid NPs were prepared by adding 5% mol ACUPA-lipid conjugates, and tumor accumulation of the NPs was evaluated by both SPECT/CT imaging and NIR fluorescence imaging and ex vivo organ biodistribution in a mice model bearing PSMA+ PC3-Pip and PSMA- PC3-Flu xenografts (Figure 6D,E). The gene discussed is FOLH1; the disease is neoplasm.