The results in in vitro cultures of the Caco-2 colon cancer cell line indicated that in comparison with free Fe-bLf, AEC-CCo-CP-Fe-bLf NCs were efficiently internalized and caused an effective inhibition of tumor spheroid growth by decreasing the expression of survivin (an apoptosis inhibitor) and the expression malignancy markers on stem cells. This evidence concerns the gene CP and colonic neoplasm.