As mentioned above, the introduction of PEG chain improved the pharmacokinetics and bioavailability of [18F]LG-1 and overcame the drawbacks of polarity and charge of [18F]LN [29,35], consequently generating better tumor imaging than [18F]LN, while the more lipophilicity and weaker PD-L1 binding affinity contributed to the poorer PET images than [18F]LG-1. This evidence concerns the gene CD274 and neoplasm.