In another study, withaferin A treatment in a liver cancer nude mouse model decreased macrophage infiltration and the inhibition of protein tyrosine kinase-2 (Pyk2), Rho-associated kinases 1(ROCK1), and vascular endothelial growth factor (VEGF) expression, along with cancer tissue necrosis and actin suppression. The gene discussed is VEGFA; the disease is liver cancer.