To determine whether astrocytes have altered expression of mitochondrial OXPHOS subunits in Alpers’ syndrome, the intensity of NDUFB8 and COXI immunofluorescence signals, normalised to porin, were quantified within GFAP + astrocytes in occipital cortex tissues from six patients with Alpers’ syndrome [45]. Here, MT-CO1 is linked to Alpers syndrome.