To evaluate the in vivo tumor targeting and biodistribution of BsAb-armed T cells, we separately labeled a near-infrared fluorescent probe (NIR-797) on various anti-PSMA/anti-CD3 BsAb-armed T cells or conventional T cells (OKT3-T cells), and then compared the tumor targeting efficiency and biodistribution of these T cells in mice bearing LNCaP tumors by using an optical imaging system. This evidence concerns the gene FOLH1 and neoplasm.