Unexpectedly, T2DM subjects had significantly higher serum TSP-2 levels than healthy [7.03 (3.48–12.91) vs. 6.86 (5.11–8.36) ng/mL, p = 0.014], in addition, the levels were significantly increased in these T2DM patients with early DKD compared to those without [10.48 (7.19–15.73) vs. 7.03 (3.48–12.91) ng/mL, p < 0.001], suggesting that TSP-2 may be related to the progress of early DKD in these patients with T2DM. This evidence concerns the gene THBS2 and type 2 diabetes mellitus.