To improve the targeting of tumor cells expressing PD-L1 and reduce possible toxicity, the binding affinity of HK010 to human 4-1BB was designed to be lower than that to human PD-L1 (KD: 493 nM versus 2.27 nM), ensuring a preferential distribution of HK010 to PD-L1-expressing cells in the TME to locally stimulate antigen-specific T cells. The gene discussed is CD274; the disease is neoplasm.