In addition, the correlation of five signature genes with the infiltration abundance of common tumor immune cells was analyzed based on TCGA HCC mRNA-seq data in present study, and the results showed that LY6E showed a significant positive correlation with immune cells such as Tem, NK CD56bright cells, Macrophages (r > 0.4, P < 0.001, Fig. 8d). This evidence concerns the gene LY6E and hepatocellular carcinoma.