The interaction of titin and MyBP-C is of special interest as MyBP-C plays an important role in modulating the acto-myosin interaction during muscle contraction and its mutation is critically associated with heart disease, being responsible for ~ 40% of all known mutations leading to hypertrophic cardiomyopathy (Heling et al. 2020). This evidence concerns the gene MYBPC3 and hypertrophic cardiomyopathy.