In this study, we used Myo6C442Y point mutation mice and MYO6 knock-out mice as experimental animals, combined with molecular biology and whole-cell patch clamp technology to explore the physiological and pathological mechanisms of MYO6 gene mutation or deletion on auditory development and mutation-induced deafness, thus providing a certain experimental basis for improving and treating non-syndromic deafness. Here, MYO6 is linked to deafness.