Herein, we infected Raw264.7 macrophages with BCG at MOI 10 and detected the expression levels of mmu-miR-25-3p, DUSP10 mRNA, and DUSP10 protein by qRT-PCR and Western blotting at 0, 1, 4, 8, 12, 24, 48, and 72 h post-infection. The gene discussed is DUSP10; the disease is infection.