To investigate the possible tumour suppressive properties of ZNF154 and ZNF132 proteins in head and neck cancer, we attempted to overexpress these proteins in a head and neck squamous cell carcinoma cell line (UM-SCC-1) by lentiviral transduction of a C-terminal Flag-tagged fusion protein construct under the control of a CMV promoter. Here, ZNF154 is linked to head and neck squamous cell carcinoma.