APP and Alzheimer disease: Elevating SIRT1 protein levels using various methods, including the small molecule A03, AAV-mediated SIRT1 overexpression, and induced SIRT1 expression, have been shown to have protective effects in an AD mouse model [182], N2a cells transfected with amyloid precursor protein [183], RGC neurons [184], and high-fat diet-induced diabetic neuropathy [185].