To determine whether the activity of grafted cerebral organoids in stroke mice is necessary for sensorimotor restoration after stroke, we prepared the cerebral organoids from IMR-90–4 iPSCs transduced with rLV-EF1a-hM4D(Gi)-mCherry-WRPE, a recombinant virus vector that silences the activity of infected neurons in the presence of designer drugs (DREADDs) agonist clozapine-N-oxide (CNO), and transplanted them into the junction of the infarct core and the peri-infarct zone of stroke mice. This evidence concerns the gene GNAI1 and stroke disorder.