Also, the crawling speeds of TCR transgenic P14 and CL4 CD8+ T cells, whose cognate antigen (P14) is cleared or matching MHC class I (CL4) is absent in our experimental setup, were found comparable in spinal cord microvessels in ODC-OVA or WT C57BL/6 J mice at day 7 after LMCV-OVA infection (Fig. 7F), further underscoring that MHC class I-restricted recognition of cognate Ag reduces CD8+ T cell crawling speed on the inflamed BBB in vivo. Here, CD8A is linked to infection.