Our results show that PFKFB3 expression was correlated positively with TMB in THYM, ACC, COAD, SARC, and skin cutaneous melanoma (SKCM); and correlated negatively with TMB in LUSC, BRCA, brain lower grade glioma (LGG), THCA, PRAD, LIHC, and UVM (Figure 3C). The gene discussed is PFKFB3; the disease is cutaneous melanoma.