Combining CIBERSORT and TIMER analysis, we found a significant positive association between PFKFB3 expression level and neutrophil, macrophage, and myeloid dendritic cells infiltration in pan-cancer (Figure 4A and Supplementary Figure 6), and a negative correlation between PFKFB3 expression level and NK cells and B cells infiltration in pan-cancer (Figure 4A and Supplementary Figure 6). Here, PFKFB3 is linked to cancer.