Of the samples sequenced, heterogeneity was primarily identified at aa positions 19 (T19IR and T19KR), 371 (S371FP), and 484 (E484AK, E484AQ, E484KQ), similar to the SNP PCR which identified heterogeneity of the S protein at the aforementioned aa positions, before the 3rd wave through to the 5th wave of infections in South Africa. This evidence concerns the gene PROS1 and infection.