HSPB1 knockout mice subjected to myocardial infarction (MI) exhibited enhanced and prolonged leukocyte infiltration, enhanced inflammatory cytokine expression, and enhanced toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/NF-κB activation in their heart tissue. The gene discussed is TLR4; the disease is myocardial infarction.