In addition, qRT-PCR and western blotting indicated that compared with the NC group, the mRNA and protein levels of MYLK, MYL9, MYH11, CALD1, ACTA2, SPP1, and CNN1 were differentially expressed in the PCa group, which is consistent with the analysis results of GEO datasets. The gene discussed is ACTA2; the disease is posterior cortical atrophy.