The boxplot results indicated that all the downregulated genes identified in this study, including ACTA2, FLNA, MYH11, TAGLN, LDB3, MYLK, TPM1, MYL9, CNN1, FLNC, LMOD1, SMTN, CALD1, and CAV1, were also significantly downregulated in TCGA prostate cancers, and the upregulated gene SPP1 was also found to be significantly overexpressed in TCGA prostate cancers. This evidence concerns the gene SPP1 and Familial prostate cancer.