Meanwhile, the highest positive rate of CEA and CA19-9 was only 12.50%, while the positive rate of combined CETN2 and POFUT1 autoantibodies was more than 33.33% in different stages, implying the potential value of CETN2 and POFUT1 autoantibodies for early screening and detection of early ESCC. Here, POFUT1 is linked to esophageal squamous cell carcinoma.